A novel passive microfluidic device for preprocessing whole blood for point of care diagnostics
Publication: Research - peer-review › Article in proceedings – Annual report year: 2009
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A novel passive microfluidic device for preprocessing whole blood for point of care diagnostics. / Shah, Pranjul Jaykumar; Dimaki, Maria; Svendsen, Winnie Edith.
In: International Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009.. IEEE, 2009.Publication: Research - peer-review › Article in proceedings – Annual report year: 2009
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TY - GEN
T1 - A novel passive microfluidic device for preprocessing whole blood for point of care diagnostics
A1 - Shah,Pranjul Jaykumar
A1 - Dimaki,Maria
A1 - Svendsen,Winnie Edith
AU - Shah,Pranjul Jaykumar
AU - Dimaki,Maria
AU - Svendsen,Winnie Edith
PB - IEEE
PY - 2009
Y1 - 2009
N2 - A novel strategy to sort the cells of interest (White Blood Cells (leukocytes)) by selectively lysing the Red Blood Cells (erythrocytes) in a miniaturized microfluidic device is presented. Various methods to lyse cells on a chip exist i.e. electrical, mechanical, chemical and thermal but they need integration of electrodes, traps, reservoirs, heaters, etc which is often difficult at microscale [1 – 4]. On the other hand, FACSlyse protocol uses only osmotic pressure to lyse erythrocytes allowing further isolation of leukocytes. This motivated us to develop a novel herringbone based lyser which works on the principle of mixing whole blood with pure water in time controlled manner to lyse erythrocytes osmotically on a chip.
AB - A novel strategy to sort the cells of interest (White Blood Cells (leukocytes)) by selectively lysing the Red Blood Cells (erythrocytes) in a miniaturized microfluidic device is presented. Various methods to lyse cells on a chip exist i.e. electrical, mechanical, chemical and thermal but they need integration of electrodes, traps, reservoirs, heaters, etc which is often difficult at microscale [1 – 4]. On the other hand, FACSlyse protocol uses only osmotic pressure to lyse erythrocytes allowing further isolation of leukocytes. This motivated us to develop a novel herringbone based lyser which works on the principle of mixing whole blood with pure water in time controlled manner to lyse erythrocytes osmotically on a chip.
U2 - 10.1109/SENSOR.2009.5285475
DO - 10.1109/SENSOR.2009.5285475
BT - International Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009.
T2 - International Solid-State Sensors, Actuators and Microsystems Conference, 2009. TRANSDUCERS 2009.
ER -