A Novel in Vivo Angiogenesis Mouse Model

Publication: Research - peer-reviewConference article – Annual report year: 2009

Without internal affiliation

  • Author: Wittenborn, Thomas

    SKS, Hjertemedicinsk Afdeling B

  • Author: Nygaard, Jens V.

    Aarhus University

  • Author: Horsman, Michael R.

    Aarhus University

  • Author: Vorup-Jensen, Thomas

    SKS, Hjertemedicinsk Afdeling B

  • Author: Kjems, Jorgen

    SKS, Hjertemedicinsk Afdeling B

  • Author: Thim, Troels

    SKS, Hjertemedicinsk Afdeling B

  • Author: Nielsen, Thomas

    Aarhus University

  • Author: Larsen, Esben Kjær Unmack

    Unknown

  • Author: Falk, Erling

    SKS, Hjertemedicinsk Afdeling B

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Purpose: To establish a reproducable, functional and fully vascularized angiogenesis model in mice for subsequent evaluation of leaky vessels associated with neovascularization. Materials and Methods: A biocompatible Poly-Capro-Lactone (PCL) scaffold was implanted subcutaneously in mice, and the development and progression of angiogenesis was evaluated up to 6 weeks after implantation. Mice were euthanized, sponges immersion fixed and paraffinembedded, and HE stained sections prepared for microscopic evaluation. Figure: Representative slides showing how new microvessels (angiogenesis) develop and progressively penetrate through the scaffold. The skin of the mouse is included for orientation. Results: Very little angiogenesis was seen within the scaffold after 1 week. During the subsequent weeks, the scaffold became progressively occupied by microvessel-containing new tissue. No or very limited extravasation of erythrocytes was seen. Within 5–6 weeks the implant was fully vascularized. Conclusion perspectives: We have developed a functional angiogenesis model capable of fully vascularizing the implant within 6 weeks. Histologic examination reveals few if any intra-implant hemorrhages, but Dynamic Contrast Enhanced (DCE)-MRI will elucidate if there are leaky vessels present in the scaffold. High resolution MRI and vessel density will also be assessed to fully characterize this model.
Original languageEnglish
JournalARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
Publication date2009
Volume29
Issue7
PagesE95-E95
ISSN1079-5642
StatePublished

Conference

Conference10. Annual Conference on Arteriosclerosis, Thrombosis and Vascular Biology
CityWashington DC
Period01/01/09 → …
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