A new system for higher recovery rate of water borne Cryptosporidium and Giardia

Publication: Research - peer-reviewConference abstract in proceedings – Annual report year: 2012

Standard

A new system for higher recovery rate of water borne Cryptosporidium and Giardia. / Al-Sabi, Mohammad Nafi Solaiman; Gad, J.; Berg, T.; Mogensen, T. ; Kurtzhals, J. A. L.; Enemark, Heidi L.

Joint Spring Symposium 2012 - Double burden of disease – how parasites interact with each other, their host and the society: Danish Society for Parasitology and Danish Society for Tropical Medicine & International Health. Danish Society for Parasitology, 2012. p. 10.

Publication: Research - peer-reviewConference abstract in proceedings – Annual report year: 2012

Harvard

Al-Sabi, MNS, Gad, J, Berg, T, Mogensen, T, Kurtzhals, JAL & Enemark, HL 2012, 'A new system for higher recovery rate of water borne Cryptosporidium and Giardia'. in Joint Spring Symposium 2012 - Double burden of disease – how parasites interact with each other, their host and the society: Danish Society for Parasitology and Danish Society for Tropical Medicine & International Health. Danish Society for Parasitology, pp. 10.

APA

Al-Sabi, M. N. S., Gad, J., Berg, T., Mogensen, T., Kurtzhals, J. A. L., & Enemark, H. L. (2012). A new system for higher recovery rate of water borne Cryptosporidium and Giardia. In Joint Spring Symposium 2012 - Double burden of disease – how parasites interact with each other, their host and the society: Danish Society for Parasitology and Danish Society for Tropical Medicine & International Health. (pp. 10). Danish Society for Parasitology.

CBE

Al-Sabi MNS, Gad J, Berg T, Mogensen T, Kurtzhals JAL, Enemark HL. 2012. A new system for higher recovery rate of water borne Cryptosporidium and Giardia. In Joint Spring Symposium 2012 - Double burden of disease – how parasites interact with each other, their host and the society: Danish Society for Parasitology and Danish Society for Tropical Medicine & International Health. Danish Society for Parasitology. pp. 10.

MLA

Al-Sabi, Mohammad Nafi Solaiman et al. "A new system for higher recovery rate of water borne Cryptosporidium and Giardia". Joint Spring Symposium 2012 - Double burden of disease – how parasites interact with each other, their host and the society: Danish Society for Parasitology and Danish Society for Tropical Medicine & International Health. Danish Society for Parasitology. 2012. 10.

Vancouver

Al-Sabi MNS, Gad J, Berg T, Mogensen T, Kurtzhals JAL, Enemark HL. A new system for higher recovery rate of water borne Cryptosporidium and Giardia. In Joint Spring Symposium 2012 - Double burden of disease – how parasites interact with each other, their host and the society: Danish Society for Parasitology and Danish Society for Tropical Medicine & International Health. Danish Society for Parasitology. 2012. p. 10.

Author

Al-Sabi, Mohammad Nafi Solaiman; Gad, J.; Berg, T.; Mogensen, T. ; Kurtzhals, J. A. L.; Enemark, Heidi L. / A new system for higher recovery rate of water borne Cryptosporidium and Giardia.

Joint Spring Symposium 2012 - Double burden of disease – how parasites interact with each other, their host and the society: Danish Society for Parasitology and Danish Society for Tropical Medicine & International Health. Danish Society for Parasitology, 2012. p. 10.

Publication: Research - peer-reviewConference abstract in proceedings – Annual report year: 2012

Bibtex

@inbook{8deb237e614a4fb086f836e9e3b523c1,
title = "A new system for higher recovery rate of water borne Cryptosporidium and Giardia",
publisher = "Danish Society for Parasitology",
author = "Al-Sabi, {Mohammad Nafi Solaiman} and J. Gad and T. Berg and T. Mogensen and Kurtzhals, {J. A. L.} and Enemark, {Heidi L.}",
year = "2012",
pages = "10",
booktitle = "Joint Spring Symposium 2012 - Double burden of disease – how parasites interact with each other, their host and the society",

}

RIS

TY - ABST

T1 - A new system for higher recovery rate of water borne Cryptosporidium and Giardia

A1 - Al-Sabi,Mohammad Nafi Solaiman

A1 - Gad,J.

A1 - Berg,T.

A1 - Mogensen,T.

A1 - Kurtzhals,J. A. L.

A1 - Enemark,Heidi L.

AU - Al-Sabi,Mohammad Nafi Solaiman

AU - Gad,J.

AU - Berg,T.

AU - Mogensen,T.

AU - Kurtzhals,J. A. L.

AU - Enemark,Heidi L.

PB - Danish Society for Parasitology

PY - 2012

Y1 - 2012

N2 - The two most common water borne pathogenic protozoa, Cryptosporidium and Giardia, are a common cause of outbreaks of diarrhea. Detecting these parasites in water samples depends on effective parasite recovery from the water matrix. The recovery rates of the currently used filter methods are low and the procedures are expensive and time consuming. These facts have motivated us to develop a new robust system for higher recovery of protozoan cysts from any fluid matrix. The new system consists of a sample collection chamber in which the fluid passes through a specially coated metallic filter with a carefully chosen pore size. On the reverse side of the filter a transducer delivers ultrasound waves in order to release trapped cysts and particles from the filter and break up parasite clusters. Purified Cryptosporidium oocysts and Giardia cysts were injected into the concentration unit and exposed to varying levels of ultrasonication. The concentrated parasite suspension was backwashed using air and the change in viability of the parasites was assessed by flowcytometry. Without sonication, the recovery rate of Cryptosporidium was around 2%. After short term sonication for 5, 10 or 20 seconds, the recovery rates were stable and ranging from 75-83%. While sonication is usually used for cell lyses, it can be tuned into a useful tool for enhanced separation of retinates of parasites using a specially constructed filter unit and a sonication protocol. The filtration chamber further facilitated the concentration of parasites by ensuring a backwash volume of less than 1ml. The collected parasites may be used for molecular typing and other diagnostic and research purposes. The presented design of the filter system can be used as a robust, cheap, and time effective method of isolating water borne parasites in the laboratory.

AB - The two most common water borne pathogenic protozoa, Cryptosporidium and Giardia, are a common cause of outbreaks of diarrhea. Detecting these parasites in water samples depends on effective parasite recovery from the water matrix. The recovery rates of the currently used filter methods are low and the procedures are expensive and time consuming. These facts have motivated us to develop a new robust system for higher recovery of protozoan cysts from any fluid matrix. The new system consists of a sample collection chamber in which the fluid passes through a specially coated metallic filter with a carefully chosen pore size. On the reverse side of the filter a transducer delivers ultrasound waves in order to release trapped cysts and particles from the filter and break up parasite clusters. Purified Cryptosporidium oocysts and Giardia cysts were injected into the concentration unit and exposed to varying levels of ultrasonication. The concentrated parasite suspension was backwashed using air and the change in viability of the parasites was assessed by flowcytometry. Without sonication, the recovery rate of Cryptosporidium was around 2%. After short term sonication for 5, 10 or 20 seconds, the recovery rates were stable and ranging from 75-83%. While sonication is usually used for cell lyses, it can be tuned into a useful tool for enhanced separation of retinates of parasites using a specially constructed filter unit and a sonication protocol. The filtration chamber further facilitated the concentration of parasites by ensuring a backwash volume of less than 1ml. The collected parasites may be used for molecular typing and other diagnostic and research purposes. The presented design of the filter system can be used as a robust, cheap, and time effective method of isolating water borne parasites in the laboratory.

UR - http://parasitology.dk/web/media/Spring%20symposium%20-%20program%20and%20abstracts/Abstracts%20Spring%20Symposium%202012.pdf

BT - Joint Spring Symposium 2012 - Double burden of disease – how parasites interact with each other, their host and the society

T2 - Joint Spring Symposium 2012 - Double burden of disease – how parasites interact with each other, their host and the society

SP - 10

ER -