TY - JOUR

T1 - A multiplex ligation detection assay for the characterization of Salmonella enterica strains

A1 - Aarts,Henk J.M.

A1 - Vos,Pieter

A1 - Larsson,Jonas T.

A1 - van Hoek,Angela H.A.M.

A1 - Huehn,Stephan

A1 - Weijers,Thijs

A1 - Grønlund,Hugo Ahlm

A1 - Malorny,Burkhard

AU - Aarts,Henk J.M.

AU - Vos,Pieter

AU - Larsson,Jonas T.

AU - van Hoek,Angela H.A.M.

AU - Huehn,Stephan

AU - Weijers,Thijs

AU - Grønlund,Hugo Ahlm

AU - Malorny,Burkhard

PB - Elsevier BV

PY - 2011

Y1 - 2011

N2 - A proof of principle of a multi-target assay for genotyping Salmonella has been developed targeting 62 genomic marker sequences of Salmonella related to pathogenicity. The assay is based on multiplex ligation detection reaction (LDR) followed by customized ArrayTube® microarray detection. The feasibility of the developed assay was verified in a method comparison study with conventional PCR using 16 Salmonella ‘test’ strains comprising eight serovars. Subsequently, the feasibility of the LDR microarray assay was also tested by analyzing 41 strains belonging to 23 serovars. With the exception of four serovars each serovar was characterized by a unique virulence associated gene repertoire. The LDR microarray platform proved to be a convenient, rapid and easy to use tool with potential in tracing a Salmonella contamination in the food chain, for outbreak studies, and to provide data for risk assessors that support bio-traceability models.

AB - A proof of principle of a multi-target assay for genotyping Salmonella has been developed targeting 62 genomic marker sequences of Salmonella related to pathogenicity. The assay is based on multiplex ligation detection reaction (LDR) followed by customized ArrayTube® microarray detection. The feasibility of the developed assay was verified in a method comparison study with conventional PCR using 16 Salmonella ‘test’ strains comprising eight serovars. Subsequently, the feasibility of the LDR microarray assay was also tested by analyzing 41 strains belonging to 23 serovars. With the exception of four serovars each serovar was characterized by a unique virulence associated gene repertoire. The LDR microarray platform proved to be a convenient, rapid and easy to use tool with potential in tracing a Salmonella contamination in the food chain, for outbreak studies, and to provide data for risk assessors that support bio-traceability models.

KW - Bio-traceability

KW - Microarray

KW - Pathogenicity

KW - Salmonella

KW - LDR

U2 - 10.1016/j.ijfoodmicro.2010.10.010

DO - 10.1016/j.ijfoodmicro.2010.10.010

JO - International Journal of Food Microbiology

JF - International Journal of Food Microbiology

SN - 0168-1605

VL - 145

SP - S68-S78

ER -