A multiplex ligation detection assay for the characterization of Salmonella enterica strains

Publication: Research - peer-reviewJournal article – Annual report year: 2011

  • Author: Aarts, Henk J.M.

    RIKILT-Institute of Food Safety

  • Author: Vos, Pieter

    Check-Points B.V.

  • Author: Larsson, Jonas T.

    Statens Serum Institut, Department of Bacteriology, Mycology and Parasitology

  • Author: van Hoek, Angela H.A.M.

    RIKILT-Institute of Food Safety

  • Author: Huehn, Stephan

    National Salmonella Reference Laboratory

  • Author: Weijers, Thijs

    Check-Points B.V.

  • Author: Grønlund, Hugo Ahlm

    National Food Institute, Technical University of Denmark, Mørkhøj Bygade 19, 2860, Søborg, Denmark

  • Author: Malorny, Burkhard

    National Salmonella Reference Laboratory

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A proof of principle of a multi-target assay for genotyping Salmonella has been developed targeting 62 genomic marker sequences of Salmonella related to pathogenicity. The assay is based on multiplex ligation detection reaction (LDR) followed by customized ArrayTube® microarray detection. The feasibility of the developed assay was verified in a method comparison study with conventional PCR using 16 Salmonella ‘test’ strains comprising eight serovars. Subsequently, the feasibility of the LDR microarray assay was also tested by analyzing 41 strains belonging to 23 serovars. With the exception of four serovars each serovar was characterized by a unique virulence associated gene repertoire. The LDR microarray platform proved to be a convenient, rapid and easy to use tool with potential in tracing a Salmonella contamination in the food chain, for outbreak studies, and to provide data for risk assessors that support bio-traceability models.
Original languageEnglish
JournalInternational Journal of Food Microbiology
Publication date2011
Volume145
PagesS68-S78
ISSN0168-1605
DOIs
StatePublished
CitationsWeb of Science® Times Cited: 2

Keywords

  • Bio-traceability, Microarray, Pathogenicity, Salmonella, LDR
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