Publication: Research - peer-review › Poster – Annual report year: 2012
In the current work a methodology for choosing a feasible cascade system that will remove co-product to meet process requirements under process relevant conditions will be presented. Decisions are based on thermodynamic constraints, kinetics, selectivity, stability, pH change, cascade enzyme compatibility and downstream processing. The methodology has been applied to an ω-transaminase system which is thermodynamically challenged and enzymatic ISCPR is deployed to shift the equilibrium. The enzymes proposed for co-product removal are dehydrogenases: lactate dehydrogenase (EC 184.108.40.206), alanine dehydrogenase (EC 220.127.116.11), yeast alcohol dehydrogenase (EC 18.104.22.168); pyruvate decarboxylase (EC 22.214.171.124), acetolactate synthase (EC 126.96.36.199) and as co-factor recycling enzymes: glucose dehydrogenase (EC 188.8.131.52), formate dehydrogenase (EC 184.108.40.206) and phosphite dehydrogenase (EC 220.127.116.11). The methodology gives an insight into the constraints of different cascade systems and is the basis for process set-up of selected cascades. Experimental and calculated data will be used to illustrate the methodology.
|Conference||Multistep Enzyme-Catalyzed Processes 2012|
|Period||10/04/12 → 13/04/12|
Acknowledgment is made of the project AMBIOCAS financed through the European Union 7th Framework Programme (Grant agreement no.: 245144)
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