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@article{7fea61292ae14ff38a7f549e86c834b3,
title = "A genome-wide polyketide synthase deletion library uncovers novel genetic links to polyketides and meroterpenoids in Aspergillus nidulans",
keywords = "Shamixanthone, Arugosin, Filamentous fungi, Austinol, Secondary metabolism, Violaceol",
publisher = "Wiley-Blackwell Publishing Ltd.",
author = "Nielsen, {Michael Lynge} and Nielsen, {Jakob Blæsbjerg} and Christian Rank and Klejnstrup, {Marie Louise} and Holm, {Dorte Koefoed} and Brogaard, {Katrine Husted} and Hansen, {Bjarne Gram} and Frisvad, {Jens Christian} and Larsen, {Thomas Ostenfeld} and Mortensen, {Uffe Hasbro}",
year = "2011",
doi = "10.1111/j.1574-6968.2011.02327.x",
volume = "321",
number = "2",
pages = "157--166",
journal = "F E M S Microbiology Letters",
issn = "0378-1097",

}

RIS

TY - JOUR

T1 - A genome-wide polyketide synthase deletion library uncovers novel genetic links to polyketides and meroterpenoids in Aspergillus nidulans

A1 - Nielsen,Michael Lynge

A1 - Nielsen,Jakob Blæsbjerg

A1 - Rank,Christian

A1 - Klejnstrup,Marie Louise

A1 - Holm,Dorte Koefoed

A1 - Brogaard,Katrine Husted

A1 - Hansen,Bjarne Gram

A1 - Frisvad,Jens Christian

A1 - Larsen,Thomas Ostenfeld

A1 - Mortensen,Uffe Hasbro

AU - Nielsen,Michael Lynge

AU - Nielsen,Jakob Blæsbjerg

AU - Rank,Christian

AU - Klejnstrup,Marie Louise

AU - Holm,Dorte Koefoed

AU - Brogaard,Katrine Husted

AU - Hansen,Bjarne Gram

AU - Frisvad,Jens Christian

AU - Larsen,Thomas Ostenfeld

AU - Mortensen,Uffe Hasbro

PB - Wiley-Blackwell Publishing Ltd.

PY - 2011

Y1 - 2011

N2 - Fungi possess an advanced secondary metabolism that is regulated and coordinated in a complex manner depending on environmental challenges. To understand this complexity, a holistic approach is necessary. We initiated such an analysis in the important model fungus Aspergillus nidulans by systematically deleting all 32 individual genes encoding polyketide synthases. Wild-type and all mutant strains were challenged on different complex media to provoke induction of the secondary metabolism. Screening of the mutant library revealed direct genetic links to two austinol meroterpenoids and expanded the current understanding of the biosynthetic pathways leading to arugosins and violaceols. We expect that the library will be an important resource towards a systemic understanding of polyketide production in A. nidulans.

AB - Fungi possess an advanced secondary metabolism that is regulated and coordinated in a complex manner depending on environmental challenges. To understand this complexity, a holistic approach is necessary. We initiated such an analysis in the important model fungus Aspergillus nidulans by systematically deleting all 32 individual genes encoding polyketide synthases. Wild-type and all mutant strains were challenged on different complex media to provoke induction of the secondary metabolism. Screening of the mutant library revealed direct genetic links to two austinol meroterpenoids and expanded the current understanding of the biosynthetic pathways leading to arugosins and violaceols. We expect that the library will be an important resource towards a systemic understanding of polyketide production in A. nidulans.

KW - Shamixanthone

KW - Arugosin

KW - Filamentous fungi

KW - Austinol

KW - Secondary metabolism

KW - Violaceol

U2 - 10.1111/j.1574-6968.2011.02327.x

DO - 10.1111/j.1574-6968.2011.02327.x

JO - F E M S Microbiology Letters

JF - F E M S Microbiology Letters

SN - 0378-1097

IS - 2

VL - 321

SP - 157

EP - 166

ER -