A detailed investigation of the porcine skin and nose microbiome using universal and Staphylococcus specific primers

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A detailed investigation of the porcine skin and nose microbiome using universal and Staphylococcus specific primers. / Strube, Mikael Lenz; Hansen, Julie Elvekjær; Rasmussen, Sophia; Pedersen, Karl.

In: Scientific Reports, Vol. 8, 12751, 2018.

Research output: Contribution to journalJournal article – Annual report year: 2018Researchpeer-review

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@article{8e1be8e5a88f45d6aff4f17284867957,
title = "A detailed investigation of the porcine skin and nose microbiome using universal and Staphylococcus specific primers",
abstract = "MRSA is an increasing problem in humans as well as livestock. The bacterial co-colonization of the skin in MRSA carriers has been poorly investigated and moreover, there have been no methods for high resolution investigations of the Staphylococcus genus apart from tediously culturing or doing multiple PCRs. On 120 samples from pig ear, skin and nose, we generated amplicons from the V1-V2 region of the 16S rRNA gene to gather an overview of the genus-level microbiome, along with using MRSA specific plates to count MRSA. In parallel with this, amplicons of the tuf gene were generated, targeting only a region of the tuf gene found only in the Staphylococcus genus. Using these methods, we determined a core microbiota across the healthy pig and determined the Staphylococcus genus to be dominated by S. equorum. Moreover, we found Streptococcus to be inversely associated with Staphylococcus and MRSA, suggesting a role for this genus in combating MRSA. In this work, we have thoroughly investigated the skin and nose microbiome of the pig and developed a high throughput method for profiling the Staphylococcus genus which we believe will be useful for further investigations.",
author = "Strube, {Mikael Lenz} and Hansen, {Julie Elvekj{\ae}r} and Sophia Rasmussen and Karl Pedersen",
note = "Open Access This article is licensed under a Creative Commons Attribution 4.0 International License",
year = "2018",
doi = "10.1038/s41598-018-30689-y",
language = "English",
volume = "8",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

RIS

TY - JOUR

T1 - A detailed investigation of the porcine skin and nose microbiome using universal and Staphylococcus specific primers

AU - Strube, Mikael Lenz

AU - Hansen, Julie Elvekjær

AU - Rasmussen, Sophia

AU - Pedersen, Karl

N1 - Open Access This article is licensed under a Creative Commons Attribution 4.0 International License

PY - 2018

Y1 - 2018

N2 - MRSA is an increasing problem in humans as well as livestock. The bacterial co-colonization of the skin in MRSA carriers has been poorly investigated and moreover, there have been no methods for high resolution investigations of the Staphylococcus genus apart from tediously culturing or doing multiple PCRs. On 120 samples from pig ear, skin and nose, we generated amplicons from the V1-V2 region of the 16S rRNA gene to gather an overview of the genus-level microbiome, along with using MRSA specific plates to count MRSA. In parallel with this, amplicons of the tuf gene were generated, targeting only a region of the tuf gene found only in the Staphylococcus genus. Using these methods, we determined a core microbiota across the healthy pig and determined the Staphylococcus genus to be dominated by S. equorum. Moreover, we found Streptococcus to be inversely associated with Staphylococcus and MRSA, suggesting a role for this genus in combating MRSA. In this work, we have thoroughly investigated the skin and nose microbiome of the pig and developed a high throughput method for profiling the Staphylococcus genus which we believe will be useful for further investigations.

AB - MRSA is an increasing problem in humans as well as livestock. The bacterial co-colonization of the skin in MRSA carriers has been poorly investigated and moreover, there have been no methods for high resolution investigations of the Staphylococcus genus apart from tediously culturing or doing multiple PCRs. On 120 samples from pig ear, skin and nose, we generated amplicons from the V1-V2 region of the 16S rRNA gene to gather an overview of the genus-level microbiome, along with using MRSA specific plates to count MRSA. In parallel with this, amplicons of the tuf gene were generated, targeting only a region of the tuf gene found only in the Staphylococcus genus. Using these methods, we determined a core microbiota across the healthy pig and determined the Staphylococcus genus to be dominated by S. equorum. Moreover, we found Streptococcus to be inversely associated with Staphylococcus and MRSA, suggesting a role for this genus in combating MRSA. In this work, we have thoroughly investigated the skin and nose microbiome of the pig and developed a high throughput method for profiling the Staphylococcus genus which we believe will be useful for further investigations.

U2 - 10.1038/s41598-018-30689-y

DO - 10.1038/s41598-018-30689-y

M3 - Journal article

VL - 8

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 12751

ER -