A CBM20 low-affinity starch-binding domain from glucan, water dikinase

Publication: Research - peer-reviewJournal article – Annual report year: 2009

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A CBM20 low-affinity starch-binding domain from glucan, water dikinase. / Christiansen, Camilla; Abou Hachem, Maher; Glaring, M.A.; Vikso-Nielsen, A.; Sigurskjold, B.W.; Svensson, Birte; Blennow, A.

In: FEBS Letters, Vol. 583, No. 7, 2009, p. 1159-1163.

Publication: Research - peer-reviewJournal article – Annual report year: 2009

Harvard

Christiansen, C, Abou Hachem, M, Glaring, MA, Vikso-Nielsen, A, Sigurskjold, BW, Svensson, B & Blennow, A 2009, 'A CBM20 low-affinity starch-binding domain from glucan, water dikinase' FEBS Letters, vol 583, no. 7, pp. 1159-1163., 10.1016/j.febslet.2009.02.045

APA

CBE

Christiansen C, Abou Hachem M, Glaring MA, Vikso-Nielsen A, Sigurskjold BW, Svensson B, Blennow A. 2009. A CBM20 low-affinity starch-binding domain from glucan, water dikinase. FEBS Letters. 583(7):1159-1163. Available from: 10.1016/j.febslet.2009.02.045

MLA

Vancouver

Author

Christiansen, Camilla; Abou Hachem, Maher; Glaring, M.A.; Vikso-Nielsen, A.; Sigurskjold, B.W.; Svensson, Birte; Blennow, A. / A CBM20 low-affinity starch-binding domain from glucan, water dikinase.

In: FEBS Letters, Vol. 583, No. 7, 2009, p. 1159-1163.

Publication: Research - peer-reviewJournal article – Annual report year: 2009

Bibtex

@article{7c6de35c899b42a087b4bc45b5b4987d,
title = "A CBM20 low-affinity starch-binding domain from glucan, water dikinase",
keywords = "Carbohydrate-binding module 20, Starch-binding domain, Surface plasmon resonance, Glucan, water dikinase, Bioimaging",
publisher = "Elsevier BV",
author = "Camilla Christiansen and {Abou Hachem}, Maher and M.A. Glaring and A. Vikso-Nielsen and B.W. Sigurskjold and Birte Svensson and A. Blennow",
year = "2009",
doi = "10.1016/j.febslet.2009.02.045",
volume = "583",
number = "7",
pages = "1159--1163",
journal = "FEBS Letters",
issn = "0014-5793",

}

RIS

TY - JOUR

T1 - A CBM20 low-affinity starch-binding domain from glucan, water dikinase

A1 - Christiansen,Camilla

A1 - Abou Hachem,Maher

A1 - Glaring,M.A.

A1 - Vikso-Nielsen,A.

A1 - Sigurskjold,B.W.

A1 - Svensson,Birte

A1 - Blennow,A.

AU - Christiansen,Camilla

AU - Abou Hachem,Maher

AU - Glaring,M.A.

AU - Vikso-Nielsen,A.

AU - Sigurskjold,B.W.

AU - Svensson,Birte

AU - Blennow,A.

PB - Elsevier BV

PY - 2009

Y1 - 2009

N2 - The family 20 carbohydrate-binding module (CBM20) of the Arabidopsis starch phosphorylator glucan, water dikinase 3 (GWD3) was heterologously produced and its properties were compared to the CBM20 from a fungal glucoamylase (GA). The GWD3 CBM20 has 50-fold lower affinity for cyclodextrins than that from GA. Homology modelling identified possible structural elements responsible for this weak binding of the intracellular CBM20. Differential binding of fluorescein-labelled GWD3 and GA modules to starch granules in vitro was demonstrated by confocal laser scanning microscopy and yellow fluorescent protein-tagged GWD3 CBM20 expressed in tobacco confirmed binding to starch granules in planta.

AB - The family 20 carbohydrate-binding module (CBM20) of the Arabidopsis starch phosphorylator glucan, water dikinase 3 (GWD3) was heterologously produced and its properties were compared to the CBM20 from a fungal glucoamylase (GA). The GWD3 CBM20 has 50-fold lower affinity for cyclodextrins than that from GA. Homology modelling identified possible structural elements responsible for this weak binding of the intracellular CBM20. Differential binding of fluorescein-labelled GWD3 and GA modules to starch granules in vitro was demonstrated by confocal laser scanning microscopy and yellow fluorescent protein-tagged GWD3 CBM20 expressed in tobacco confirmed binding to starch granules in planta.

KW - Carbohydrate-binding module 20

KW - Starch-binding domain

KW - Surface plasmon resonance

KW - Glucan, water dikinase

KW - Bioimaging

U2 - 10.1016/j.febslet.2009.02.045

DO - 10.1016/j.febslet.2009.02.045

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 7

VL - 583

SP - 1159

EP - 1163

ER -