Martin Dufva

Martin Dufva

Associate Professor

Produktionstorvet, Building 423, Room 024

2800 Kgs. Lyngby

Denmark

Phone: 45256324

Mobile: 5133 3753

Fax: 4588 7762

CURRICULUM VITAE

Associate Professor Dr. Martin Dufva,

 

Personel Data           

Martin Dufva, Swedish citizen born Nov-11 1967, married 1994 and has three children, Granvænget 3, DK3100 Hornbæk tel +45 3012 8416, Working address work: Department of micro and Nanotechnology, Technical University of Denmark, DK2800 Kgs Lyngby. Tel +45 25 63 24, e-mail: martin.dufva@nanotech.dtu.dk

 

Educations 

1994            Bachelor of Science in Biology at Göteborg University

1998             Master of Science in molecular biology at Göteborg University.

2001            Ph D (Med. Dr.) in Clinical chemistry and transfusion medicine, Göteborg University

 

Positions

1987-1990: Atlas Resors computer department.

1991-2001: Computer consultant for SIXU föreningsservice AB.

1995-2001: PhD students at clinical chemistry and transfusion medicine, Göteborg University

2001-2005: Assistant Prof, Department of Micro and Nano Technology, Danish Technical University (DTU)

2005-        : Associate Prof, Department of Micro and Nano Technology, (DTU)

2002-2006:Paternity leave for 25 whole weeks and 25 on part time during the period

 

Selected Management skills:

  • Since 2005- leader of my research group at DTU Nanotech consisting of 3-8 people.
  • Medarbejder udvikling samtaler (MUS) with employees.
  • Principle leader of 8 PhD projects and nine master projects.

Supervisor for 16 PhD student project.14 Master students, 6 Bachelor projects and 17 special course projects.

 

Research focus areas:

DNA and protein microarray technology: Function of biomolecules on surfaces, immobilisation of DNA and proteins to surfaces, genotyping, antibody assays, detection technologies and clinical performance of my invention gradient processing of DNA microarrays.

Cell culture on chip: design of large combinatory assay system for high throughput cell analysis, biocompatibility issues of perfusion and programming of cells by multiple fluidic streams

Microfluidics:design fluidics networks to solve limitation in molecular and cell based assays. Often I utilize arrays of fluidics streams in combination with biochemical arrays. I seek solution that can be utilized by end users and develop fluidics components to that end including good optimal quality of chips for time laps microscopy and interconnection and pumping for simplicity and robustness.

High throughput screening of biological: This is a novel acvitivity employing microfluidics droplet technology. Curretly the screening deals with antibody,  phage diplay and bacterial promoter libraries. 

Peer reviewed journal articles (61), proceedings (28) and patents (1) Total cites 1045,  H-index:18 (19 including books). C. Non peer reviewed publications 64 (Conference abstracts: 50 (8oral), book chapters: 9, books :1, In the news:1. Others publications: 5.). Google scholar statistics: 

 

CV

Education

1992 - 1998 Msc in Molecular Biology - Göteborg University

Academic grades

M Sc Molecular Biology

PhD

Professional experience

1991 - 2001 Computer consultant - SIXU förenings service
1987 - 1990 Programmer - Atlas Resor A/B

Languages

Swedish (mother language), English, Danish (spoken)

Expertise

Research profile

The major research line is to create in vivo like condition in vitro  and to monitor, in real time, intracellular events like the regulation of key genes. Currently, the technology is used  for stem cell research. The problems investigated are to control and increase the efficiency of stem cell differentiation (mostly adult stem cells like nerve stem cells and adipocyte stem cells) and migration to sites of injury (mesenchymal stem cells). The aim of the research is to find tools based to gain new biological knowledge. The approach is integrating many fields of technology, including micro and nanotechnology, automation, surface chemistry and molecular biology. A lesser part of the research interests is microarray technology and its applications in cell function, genetics, proteomics and implementation in point of care devices. In this research line, the interests are in mechanistic insight of biomolecule/surface interactions, assay development strategies and surface chemistries.

Other information

 

 

 

Peer reviewed articles in Journals

 

 

61. Rizzi, G, Østerberg FW, Dufva M and Hansen MF. Magnetoresistive sensor for real-time single nucleotide polymorphism genotyping. Biosensors and Bioelectronics 52 (2014) 445–451

60. Heiskanen A, Coman V, Kostesha N, Sabourin D, Haslett B, Baronian K, Gorton L, Dufva M and Enmeus J. Bioelectrochemical probing of intracellular redox processes in living yeast cells-application of redox polymer wiring in a microfluidic environment. Analytical and bioanalytical chemistry. 405, 3847-3858. 2013.

59. Illeva, M and Dufva M. Oct4 mRNA-expressing cells, detected by molecular beacons, localize to the center of neurospheres during differentiation. PLoS One 2013. 10.1371/journal.pone.0073669

58. Poulsen L, Søe MJ, Møller LB, Dufva M.. Investigation of parameters that affect the success rate of microarray-based allele-specific hybridization assays., PLoS One. 2011 Mar 22;6(3):e14777. 10.1371/journal.pone.0014777.

57. Hemmingsen, M, Vedel, S, Skafte-Pedersen, P, Sabourin, D, Collas, P, Bruus, H and Dufva M. The Role of Paracrine and Autocrine Signaling in the Early Phase of Adipogenic Differentiation of Adipose-derived Stem Cells. Accepted PLOS ONE 2013. 10.1371/journal.pone.0063638

56. Vergani, M, Carminati, M, Ferrari, G ,  Landini, E, Caviglia, C ,  Heiskanen, A ,  Comminges, C,  Zor, K ,  Sabourin, D , Dufva, M ,  Dimaki, M ,  Raiteri, R ,  Wollenberger, U , Emneus, J ,  Sampietro, M . Multichannel Bipotentiostat Integrated With a Microfluidic Platform for Electrochemical Real-Time Monitoring of Cell Cultures. 2012 IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS 6 (5). 498-507   DOI: 10.1109/TBCAS.2012.2187783

55. Skafte-Pedersen, P, Sip C, Folch, A and Dufva, M. Modular microfluidic systems using reversibly attached PDMS fluid control modules. 2013. J. Micromech. Microeng. 23 055011 doi:10.1088/0960-1317/23/5/055011

54. Joanna M. Łopacińska, Jenny Emnéus, Martin Dufva. Poly(Dimethylsiloxane) (PDMS) Affects Gene Expression in PC12 Cells Differentiating into Neuronal-Like Cells. Research Article | published 03 Jan 2013 | PLOS ONE 10.1371/journal.pone.0053107

53. David Sabourin, Peder Skafte-Pedersen, Martin Jensen Søe, Mette Hemmingsen, Massimo Alberti, Vasile Coman, Jesper Petersen, Jenny Emnéus, Jörg P. Kutter, Detlef Snakenborg, Flemming Jørgensen, Christian Clausen, Kim Holmstrøm, and Martin Dufva. The MainSTREAM Component Platform: A Holistic Approach to Microfluidic System Design. Journal of Laboratory automation. 2012 Volume 18 Issue 3 June 2013 pp. 212 - 228. doi:10.1177/2211068212461445.

52. Vergani, M, Carminati, M , Ferrari, G, Landini, E, Caviglia, C, Heiskanen, A, Zor, K, Sabourin, D, Dufva, M, Raiteri, R, Emnéus, J and Sampietro, M. Multichannel Bipotentiostat Integrated with a Microfluidic Platform for Electrochemical Real-Time Monitoring of Cell Cultures. 2012 accepted, IEEE Transactions on Biomedical Circuits and Systems journal, PP(99), DOI: 10.1109/TBCAS.2012.2187783.

51. M. Skolimowski, M. Weiss Nielsen, F. Abeille, P. Skafte-Pedersen, D. Sabourin, A. Fercher, D. Papkovsky, S. Molin, R. Taboryski, C. Sternberg, M. Dufva, O. Geschke, and J. Emnéus. Modular microfluidic system as a model of cystic fibrosis airways. 2012, Biomicrofluidics, 6, 034109.

50. Lopacinska, J, et al. Cell motility, morphology, viability and proliferation response to nanotopography on silicon black, Nanoscale 2012. 4(120, 3739-3745)

49. Yi S, Perch-Nielsen I, Dufva M, Sabourin D, Bang DD, Høgberg J, Wolff A.  Direct immobilization of DNA probes on non-modified plastics by UV irradiation and integration in microfluidic devices for rapid bioassay. Anal Bioanal Chem. 2012,  402(2), 741-748.

48. Skafte-Pedersen P, Hemmingsen M, Sabourin D, Blaga F, Bruus H, Dufva M. A self-contained, programmable microfluidic cell culture system with real-time microscopy access. Biomedical microdevices (2012), 14(2), 385-399.

47. Søe M, Sabourin D, Alberti M, Holmstrøm K, Dufva M. HistoFlex – a microfluidic device providing uniform flow conditions enabling highly sensitive, reproducible and quantitative in situ hybridizations. Lab on a chip journal 2011.

46. Soe, M, Moller, j, Dufva, M and Holmstrom K. A Sensitive Alternative for MicroRNA In Situ Hybridizations Using Probes of 2 '-O-Methyl RNA plus LNA. J Histochemistry and Cytochemistry. 2011, 59(7), 661-672

45. Optimal homogenization of Perfusion flows in Microfluidic Bio-reactor. Fridolin Okkels, Martin Dufva and Henrik Bruus. 2011, PLoS One. 2011 Jan 27;6(1):e14574.     

44. Characterizing superparamagnetic bead interactions with functionalized surfaces by an immunomicroarray. Peter Durand Skottrup, Mikkel Fougt Hansen, Jacob Moresco Lange, Maria Deryabina, Winnie Edith Svendsen, Mogens Havsteen Jakobsen, Martin Dufva. 2010.2010 Oct;6(10):3936-46. Epub 2010 Apr 24

43.       Characterization of a patch-clamp microchannel array towards neuronal networks analysis. Massimo Alberti, Detlef Snakenborg, Joanna M. Lopacinska, Martin Dufva, Jörg P. Kutter. 2010. Microfluidics and Nanofluidics 9 (4-5):963-972

42.       Sabourin D, Petersen J, Dufva M. 2010. Microfluidic DNA microarrays in PMMA chips: streamlined fabrication via simultaneous DNA immobilisation and bonding activation by brief UV exposure. Biomed Microdevices. 2010 Aug;12(4):673-81.

41. Microfluidic dissolved oxygen gradient generator biochip as a useful tool in bacterial biofilm studies. Skolimowski M, Nielsen MW, Emnéus J, Molin S, Taboryski R, Sternberg C, Dufva M, Geschke O. Lab chip 2010. Lab Chip. 2010 Aug 21;10(16):2162-9. Epub 2010 Jun 2257.

40.       Sabourin D,  Snakenborg, D and Dufva M. 2010, Interconnection blocks with minimal dead volumes permitting planar interconnection to thin microfluidic devices, Microfluidics and Nanofluidics. 9(1):87-93

39. D Sabourin , M Dufva , T Jensen3 , J Kutter  and D Snakenborg. et al. 2010, One-step fabrication of microfluidic chips with in-plane, adhesive-free interconnections. J. Micromech. Microeng. 20 (3): 037001.

38.Knudsen, K.J., G.M. Nelander Holm, J.S. Krabbe, N. Listov-Saabye, B. Kiehr, M. Dufva, J.E. Svendsen, and M.B. Oleksiewicz. 2009. Driving gradual endogenous c-myc overexpression by flow-sorting: intracellular signaling and tumor cell phenotype correlate with oncogene expression. Archives of toxicology.83(12): 1061-107

37. Skafte-Pedersen, P., D. Sabourin, M. Dufva, and D. Snakenborg. 2009. Multi-channel peristaltic pump for microfluidic applications featuring monolithic PDMS inlay. Lab on a chip 9:3003-3006.

36.Sabourin D, Snakenborg D, Dufva M. Interconnection blocks: a method for providing reusable, rapid, multiple, aligned and planar microfluidic interconnections. Journal of micromechanics and microengineering. 2009;19:035021.

35.       Dufva M, Stangegaard M. Comment on "Microfluidics meets cell biology: bridging the gap by validation and application of microscale techniques for cell biological assays". Bioessays 2009;31:255.

34. Christiansen MB, Lopacinska JM, Jakobsen MH, Dufva M and Kristensen A. Polymer photonic crystal dye lasers as Optofluidic Cell Sensors. Opt Express 2009;17:2722-30.

33.       Dufva M, Petersen J, Poulsen L. Increasing the specificity and function of DNA microarrays by processing arrays at different stringencies. 2009, Anal Bioanal Chem.  95(3): 669-677

32. Petersen J, Poulsen L, Birgens H, Dufva M. Microfludic device for creating ionic strength gradients over DNA microarrays for efficient DNA melting studies and assay development. PLoS ONE 2009;4:e4808.

31. Poulsen L, Soe MJ, Snakenborg D, Møller L, Dufva M. Multi-stringency wash of partially hybridized 60-mer probes reveals that the stringency along the probe decreases with distance from the microarray surface. Nucleic Acids Res 2008;36:e132.

30.       Gabriela Blagoi, Stephan Keller,  Alicia Johansson, Anja Boisen and Martin Dufva, Functionalization of SU-8 photoresist surfaces with IgG proteins, Applied Surface Science. 2008, 255(5), 2896-2902.

29.       Gudnason, H., M. Dufva, D. Duong Bang, and A. Wolff. 2008. An inexpensive and simple method for thermally stable immobilization of DNA on an unmodified glass surface: UV linking of poly(T)10-poly(C)10-tagged DNA probes. Biotechniques 45:261-271.

28.       Lund-Olesen, T., Dufva, M., Dahl, J.A., Collas, P. and Hansen, M.F. (2008) Sensitive on-chip quantitative real-time PCR performed on an adaptable and robust platform. Biomed Microdevices. Preprint.

27.       Larsen, A.V., Poulsen, L., Birgens, H., Dufva, M. and Kristensen, A. (2008) Pinched flow fractionation devices for detection of single nucleotide polymorphisms. Lab Chip, 8, 818-821.

26.       Petersen, J., Poulsen, L., Petronis, S., Birgens, H. and Dufva, M. (2008) Use of a multi-thermal washer for DNA microarrays simplifies probe design and gives robust genotyping assays. Nucleic Acids Res, 36, e10.

25.       Poulsen, L.K., Pedersen, M.H. andDufva, M. (2007) Allergology on a chip. Clin Exp Allergy.

24.       Gudnason, H., M. Dufva, D.D. Bang, and A. Wolff. 2007. Comparison of multiple DNA dyes for real-time PCR: effects of dye concentration and sequence composition on DNA amplification and melting temperature. Nucleic Acids Res 35:e127.

23.       Lund-Olesen, T,Dufva, M, Hansen, MF, Capture of DNA in microfluidic channel using magnetic beads: increasing capture efficiency with integrated microfluidic mixer. Journal of Magnetism and Magnetic Materials. 2007.

22.       Petersen, J., Stangegaard, M., Birgens, H. and Dufva, M. (2007) Detection of mutations in the beta-globin gene by colorimetric staining of DNA microarrays visualized by a flatbed scanner. Anal Biochem, 360, 169-171.

21.       Ringdorf, M., Jensen, J.B., Dufva, M., Pedersen, L.H., Høiby, P.E., and Bang, O., Label-free detection of biomolecules using photonic crystal fiber long-period grating sensors, 2006 Optical express, 14(18): 8224-8231.

20.       Stangegaard, M., Wang, Z., Kutter, J.P., Dufva, M., Wolff, A. Whole genome expression profiling using DNA microarray for determining biocompatibility of polymeric surfaces. Molecular BioSystems, 2006, 2, 421 - 428.

19.       Stangegaard M., Dufva IH., Dufva M., “Addendum: Reverse transcription using random pentadecamer primers increases yield and quality of resulting cDNA”, Biotechniques (2006), 41(1) , 50

18.       Stangegaard, M. Petronis, S., Jørgensen, A.M., Christensen, C.B.V and Dufva, M., A Biocompatible Micro Cell Culture Chamber (µCCC) for the Culturing and On-line Monitoring of Eukaryote Cells, Lab. Chip, 2006, 6, 1045 - 1051

17.       Dufva, M, Petersen, J, Stoltenborg, M, Birgens, H, Christensen CBV. Detection of mutations using microarrays of poly(C)10-poly(T)10 modified DNA probes immobilized on agarose films. 2006. Analytical Biochemistry, 32, 188-197

16.       Stangegaard, M Dufva, IH and Dufva, M, Reverse transcription using random pentadecamer primers increases yield and quality of resulting cDNA, Biotechniques, 2006, 40(5), 649-656

15.       Petronis, S., Stangegaard, M., Christensen, C., and Dufva, M. Transparent polymeric cell culture chip with integrated temperature control and uniform media perfusionBiotechniques. 2006, 40(3), 368-375

14.       Marie, R., S. Schmid, A. Johansson, L. Ejsing, M. Nordstrom, D. Hafliger, C.B. Christensen, A. Boisen, and M. Dufva, Immobilisation of DNA to polymerised SU-8 photoresist. Biosens Bioelectron, 2006; 21: 1327-32.

13.       Dufva,M., Fabrication of high quality microarrays. Biomol Eng, 2005; 22: 173-84.

12. Smistrup, K., B.G. Kjeldsen, J.L. Reimers, M. Dufva, J. Petersen, and M.F. Hansen, On-chip magnetic bead microarray using hydrodynamic focusing in a passive magnetic separator. Lab Chip, 2005; 5: 1315-9.

11.       Dufva, M., Comment on Wong and Medrano's "Real-time PCR for mRNA quantification" BioTechniques 39:75-85 (July 2005). Biotechniques, 2005; 39: 484; discussion 484-5.

10. Martin Dufvaand Claus BV Christensen. Diagnostic and analytical application of protein arrays. Expert Review of Proteomics, 2(1), 41-48. 2005

9. Fixe, A., M. Dufva, P. Telleman, and C. Christensen, Functionalization of Poly- (methyl methacrylate) (PMMA) as a substrate for DNA microarrays. Nucleic Acids Res. 32(1): p. e9.2004.

8. Fixe, A., M. Dufva,P. Telleman, and C. Christensen, One-step immobilization of aminated and thiolated DNA onto poly(methylmethacrylate) (PMMA) substrates. Lab-on-a-chip. 4(3): p. 191-195.2004.

7.Dufva, M., S. Petronis, L. Bjerremann Jensen, C. Krag, and C. Christensen, Characterization of an inexpensive, non-toxic and highly sensitive microarray substrate. Biotechniques. 37: p. 286.2004.

6. Belleville, E., M. Dufva, J. Aamand, L. Bruun, and C. Christensen, Quantitative Microarray Pesticide Analysis. Journal of Immunological methods. 86(1-2): p. 219-229.2004.

5. Han, A., M. Dufva, E. Belleville, and C. Christensen, Detection of analyte binding to microarrays using gold nano particles labels and a desktop scanner. Lab. Chip. 3: p. 336 - 339.2003.

4. Belleville, E., M. Dufva, J. Aamand, L. Bruun, and C. Christensen, Quantitative Assessment of Factors affecting the Sensitivity of a Competitive Immuno-Microarray for Pesticide Detection. Biotechniques. 35: p. 1044-1051.2003.

3.Dufva, M., J. Flodin, A. Nerstedt, U. Ruetschi, and L. Rymo, Epstein-Barr virus nuclear antigen 5 inhibits pre-mRNA cleavage and polyadenylation. Nucleic Acids Res. 30(10): p. 2131-43.2002.

2.Dufva, M., M. Olsson, and L. Rymo, Epstein-Barr virus nuclear antigen 5 interacts with HAX-1, a possible component of the B-cell receptor signalling pathway. J Gen Virol. 82(Pt 7): p. 1581-7.2001.

1.Dufva, M., A. Svenningsson, and G.K. Hansson, Differential regulation of macrophage scavenger receptor isoforms: mRNA quantification using the polymerase chain reaction. J Lipid Res. 36(11): p. 2282-90.1995.

 

Peer reviewed proceedings

27.  Rizzi G, Østerberg P, Dufva M and Hansen MF. Single nucleotide polymorphism (SNP) detection on a magnetoresistive sensor. MicroTAS Freiburg, Germany, 2013.

26.  Muhanty S, et al, Novel scalable silicone elastomer and poly(2-hydroxyethyl methacrylate) (PHEMA) composite materials for tissue engineering and drug delivery. International conference on biomedical engineering, Singapore 2013.

25.  Muhammad et al, . Development of a lab-on-a-chip system with integrated sensors for 3D tissue engineering applications. International conference on biomedical engineering, Singapore 2013.

24.  Canali C et al, . A bioimpedance-based sensing system for monitoring cellular dynamics in a 3D culture environment. International conference on biomedical engineering, Singapore 2013.

23.  Poulsen CE, Bang D, Dufva M, Wolff A. An injection moulded microfluidic chip for polymerase chain reaction (OCR) therocycling and imaging of droplets to detect food-bourne pathogens Camoylobacter spp. International conference on biomedical engineering, Singapore 2013.

22.  W. H. Minhass, P. Pop, J. Madsen, M. Hemmingsen, P. Skafte-Pedersen, and M. Dufva, Cell Culture Microfluidic Biochips: Experimental Throughput Maximization, In The 5th International Conference on Bioinformatics and Biomedical Engineering (iCBBE 2011). ISSN 2151-7614, Page 1-6.

21.       A microfluidic platform for personalized cancer diagnostics by padlock probes ligation and circle to circle amplification. A. Ahlford, A. J. Conde, D. Sabourin, J. P. Kutter, M. Nilsson, M. Dufva and M. Brivio. MicroTAS 2011, Seattle USA.

20. Perfusion cell culture reveals a paracrine or autocrine signalling pathways involved in adipose-derived stem cell differentiation into adipocytes. Mette Hemmingsen, Peder Skafte-Pedersen, David Sabourin, Rasmus Find Andersen, Anita L. Sørensen, Philippe Collas, and Martin Dufva. MicroTAS 2011, Seattle USA

19.       Real-time monitoring of cellular dynamics using microfluidic cell culture system with integrated electrode array potentiostat. K. Zόr1, M. Vergani, A. Heiskanen, E. Landini, M. Carminati, V. Coman, I. Vedarethinam, P. Skafte-Pedersen, M. Skolimowski, A. Martinez Serrano, M. Kokaia, T. Ramos Moreno, A. Ghio4, W.E. Svendsen, M. Dimaki, Zs. Keresztes, M. Adamovski, U. Wollenberger, D. Sabourin,

G. Ferrari, R. Raiteri, M. Sampietro, M. Dufva and J. Emnéus. MicroTAS 2011, Seattle USA.

18.       Microfluidic model of cystic fibrosis bronchi.M. Skolimowski1, M. W. Nielsen, F. Abeille, J. Lopacinska, S. Molin, R. Taboryski, O. Geschke, C. Sternberg, M. Dufva and J. Emnéus. MicroTAS 2011, Seattle USA.

17.       Modular control system for real-time on-chip generation and routing of temporal concentration gradients in microfluidic chips. A.J. Conde, D. Sabourin, P. Skafte-Pedersen and M. Dufva. MicroTAS 2011, Seattle USA.

16.       Heiskanen A, Dufva M, Emneus J. Chip based electoanalytical systems for monitoring cellular dynamics , Conference of the NATO-Advanced-Study-Institute on Microfluidics Based Microsystems - Fundamentals and Applications, Aug 23-set 04, 2009 Cesme Izmir, Turkey. Proceedings published in Microfluidics based microsystems: fundamentals and applications. Book Series: NATO Science for Peace and Security Series A-Chemistry and Biology   Pages: 399-426   Published: 2010

15. Heiskanen A, Emneus J, Dufva M, Perfusion cell culture chips. Conference of the NATO-Advanced-Study-Institute on Microfluidics Based Microsystems - Fundamentals and Applications, AUG 23-SEP 04, 2009 Cesme Izmir, TURKEY. 04, 2009 Cesme Izmir, Turkey, Proceeding published in Microfluidics based microsystems: fundamentals and applications. Book Series: NATO Science for Peace and Security Series A-Chemistry and Biology   Pages: 427-452   Published: 2010

14.       Poulsen L, Jensen Søe M, Møller L, and Dufva M. Investigation of parameters that affect the success rate of microarray-based allele-specific hybridization assays. PLoS One. 2011. 6 (3): e14777

13.Sabourin, D., Snakenborg, D., Skafte-Pedersen, P., Kutter, J.P., and Dufva, M. “Fast and Simple: Reconfigurable Elements and Solutions for Creating and Driving Fluidic Networks”, Oral presentation at µTAS 2010, Gronnigen, Netherlands.

12. Skafte-Pedersen, P., Sabourin, D., Hemmingsen, M., Østergaard, P.F., Blaga, F.S., and and Dufva, M. “A user-friendly, self-contained, programmable microfluidic cell culture system for high quality microscopy”, µTAS 2010, Gronnigen, Netherlands.

11. System-Level Modeling and Simulation of the Cell Culture Microfluidic Biochip ProCell. Wajid Hassan Minhass, Paul Pop, Jan Madsen, Mette Hemmingsen, Martin Dufva. 2010.Proceedings of the Design, Test, Integration and Packaging of MEMS/MOEMS, 5-7 May 2010, Seville, Spain

10.       Søe M, Holmstrøm K, Petersen J, Okkels F and Dufva M, Flow profiles effect on microarray hybridization, 2009, MicroTAS, Korea

9.  Skolimowski M, Weiss Nielsen M, Emnéus J, Molin S, Taboryski R, Dufva M, Sternberg C and Geschke O. Microfluidic biocip as a model of the airways of cystic fibrosis. 2009, MicroTAS Korea

8. Christiansen, MB; Lopacinska, JM.; Jakobsen, MH; Mortensen, A Dufva, M; Kristensen, A, Polymer photonic crystal dye lasers as label free evanescent cell sensors, Nanoengineering: fabrication, Properties, Optics and Devices. San Diego, CA, USA, 2009 Proceedings of SPIE, the International Society for Optical Engineering, vol: 7402 (2009).

7. Alberti, Massimo; Snakenborg, Detlef; Lopacinska, Joanna M.; Dufva, Martin; Kutter, Jörg PeterElectrochemical impedance spectroscopy for characterization of a patc clamp microchannel array and detection of cells, Presented at: The 13th International Conference on miniaturized Systems for Chemistry and life Science (MicroTAS). Jeju, South Korea, 2009

6.  Development of a microfluidic on-line culture system for combined elecrocemical and optical real-time detection of cellular processes. Arto Heiskanen, Christer Spégel, Jan Tønnesen, Zdenka Fohlerova, Merab Kokaia, Tautgirdas Ruzgas, Martin Dufva and Jenny Emnéus,  MicroTAS 2008, San Diego USA

5.  Microfluidic processing of diagnostic and commercial microarryas. Jesper Petersen, Lena Poulsen, Henrik S. Birgens and Martin Dufva, MicroTAS 2008, San Diego USA

4. Modular Microfluidic System featuring Cast PDMS Pumping Bed and Planar PDMS Interconnection Blocks. David Sabourin, Detlef Snakenborg, Peder- Skafte-Pedersen, Jörg P. Kutter and Martin Dufva MicroTAS 2008, San Diego USA.

3.  A simple hydrophilic treatment of  SU-8 surfaces for cell culturing and cell patterning. Zhenyu Wang, Michael Stangegaard, Martin Dufva, Jörg P. Kutter, and Anders Wolff. microTAS 2005. Boston USA.

2.  Petronis, S, Gantzhorn, D, Christensen CBV and Dufva, M. Multithermal DNA Micro-Array Chip for Rapid DNA Melting Temperature Measurement and Advanced SNP Discrimination. MEMS2005

1. A micro cell culture chamber (µCCC) for the culturing and study of eukaryotic cells. Michael Stangegaard, Sarunas Petronis, Claus B. V. Christensen and Martin Dufva. Screening Europe 2005. Genevé. Switzerland

 

 

Patents:

1.  Dufva, M., Petronis, S. and Petersen, J.  patent no. WO2007062666-A1.

 

Non peer reviewed publications: 61

 

Non-peer reviewed journal contributions (5)

 

5.      Lund-Olesen, T, Dufva, M, Hansen, M. Real-Time PCR in Lab-on-a-chip (2008) Bioforum Europe Highlight Issue, 12, 48-50

4.      Lund-Olesen, T, Dufva, M, Hansen, M. Real-Time PCR in Lab-on-a-chip (2007) Bioforum Europe, 11, 30-32.

3.      Lund-Olesen, T, Dufva, M, Hansen, M. Real-Time PCR in Lab-on-a-chip (2007) G.I.T Laboratory Journal Europe, 11, 30-32.

2.      Dufva, M. Production of DNA microarrays for biomedical research. Biotech International (feb/march 2006) 1-4.

1.      Dufva, M. Open access will deter illegal file-sharing (2003) Nature, 426,15.

 

In the news (1).

En lever af plastic og stamceller, Politiken, 8/7-2012, Side 8:e.

.

 Conference abstracts: 60(Oral presentations:8, invited speaker:4)

 

50. Microfluidics Component Library Enabling Microfluidic Parallel Network Automation and Control. Maciej Skolimowski. David Sabourin, Peder Skafte-Pedersen, Mette Hemmingsen, Alvaro Conde, Martin Søe, Kim Holmstrøm, Martin Dufva. 2012 IEEE-EMBS Micro and Nanotechnology in Medicine Conference Hawaii, USA.

49. Microfluidic System for 3D Modeling of the Migration Path Taken by Stem Cells in Blood to Injured Tissue. Maciej Skolimowski*, Rikke K. Andersen, Moustapha Kassem, Flemming Jørgensen, Christian Clausen, Martin Dufva. 2012 IEEE-EMBS Micro and Nanotechnology in Medicine Conference Hawaii, USA.

48. Nanomachine Based Optical Sensors. Paolo Della Vedova, Mirolyuba Ilieva, Ole Hansen, Martin Dufva. 2012 IEEE-EMBS Micro and Nanotechnology in Medicine Conference Hawaii, USA.

47. Molecular Beacon Technology Based Sensor for Real-Time Detection and Tracking Neural Stem Cell Differentiation. Mirolyuba Ilieva, Paolo Della Vedova, Martin Dufva. 2012 IEEE-EMBS Micro and Nanotechnology in Medicine Conference Hawaii, USA.

46. Microfluidic monitoring of programmed cell death in living plant tissue. Mark, C, Heiskanen, A, Zor, K,  Svensson, B, Emnéus, J, Dufva, M and Finnie, C. 2012, Plant Biology Congress, Freiburg, Germany

45.  Manipulation of the extracellular matrix microenvironment by micro-and nanotechnology approaches to improve the generation of pancreatic endocrine cells from human embryonic stem cells. Rasmussen, CH, DR, Pedersen, Dufva, M and Hansson M. International Society for Stem Cell Research 10th annual meeting. 2012, Yokohama, Japan.

44.  Manipulation of the extracellular matrix microenvironment by micro-and nanotechnology approaches to improve the generation of pancreatic endocrine cells from human embryonic stem cells. Rasmussen, CH, DR, Pedersen, Dufva, M and Hansson M.Copenhagen, Bioscience conferences- The stem cell niche – development and disease. 2012, Hillerød , DK.

43.Microfluidics tools to study paracrine signalling. Martin Dufva. Copenhagen, Bioscience conferences- The stem cell niche – development and disease. 2012, Hillerød , DK.

42. Microfluidics Systems for Enhancing Life Science Applications such as in situ Hybridization and in vitro Stem Cell Cultures. Martin Dufva. European Lab on a chip conference, 2012, Edinburgh UK.

41. Modular control system for real-time on-chip generation and routing of temporal concentration gradients in microfluidic chips. Alvaro José Conde, David Sabourin, Peder Skafte-Pedersen and Martin Dufva. 2011 Hamburg. Germany

40. Modular control system for real-time on-chip generation and routing of temporal concentration gradients in microfluidic chips. Alvaro José Conde, David Sabourin, Peder Skafte-Pedersen and Martin Dufva. 2011 Hamburg. Germany

39. Control of near-cell environment by perfusion cell culture reveals a paracrine or autocrine signaling pathway involved in adipose-derived stem cell differentiation into adipocytes. Mette Hemmingsen, Peder Skafte-Pedersen, David Sabourin, Rasmus Find Andersen, Anita L. Sørensen, Philippe Collas, and Martin Dufva. Lab-on-a-chip European conference. 2011 Hamburg. Germany

38. Immobilization of DNA on Unmodified Plastics by UV Linking, Yi Sun et al. Lab-on-a-chip European conference. 2011 Hamburg. Germany

37. Søe, M.J.; Dufva, M. and Holmstrøm, K. HistoFlex – introducing a novel biomarker analysis concept in histology Poster presentation, Biomarker World Congress, Philadelphia, USA, 2010.

36. Rindorf, Lars Henning; Jensen, Jesper Bo; Dufva, Hans Martin; Pedersen, Lars Hagsholm; Hoiby, Poul E.; Bang, Ole, Photonic crystal fiber gratings: prospects for label-free biosensors, Bragg Gratings, Photosensitivity and Poling in Glass Waveguides (BGPP) Topical Meeting. Quebec, Canada, 2007

35. Modular System for Connecting and Actuating Parallel Microfluidic Networks, David Sabourin, Peder Skafte-Pederse, Jorg Kutter, Detlef Snakenborg, Martin Dufva, Labautomation 2010, Palm Spring 2010

34. Electrical and Fluidic Characterization of a Novel Integrated System for Automatic Patch-Clamp on cultured Neural Networks, Massimo Alberti, Detlef Snakenborg, Joanna M. Lopacinska, Martin Dufva, Jörg P. Kutter. Labautomation 2010, Palm Springs, CA.

33. Dufva M et al.Methods to increase specificity of DNA hybridization assays, Biosensors 2010. Glasgow

32. Dufva M et al. Using high throughput probe selection strategies to obtain ideal allele specific hybridization probes, Advances in Microarray Technology. 2010, Dublin

31. Dufva M, Spatial Thermal or Ionic Strength Gradients over DNA Microarray Give Specific and Flexible DNA Microarrays Assays, Microarray World Congress. 2009, San Francisco

30. Sabourin D, Petersen J, Dufva M, Genotyping of Human Beta-Globin Mutations using capillary driven flows in PMMA microfluidic chips containing fully enclosed allele specific hybridization microarray, Molecular Diagnostics Europe, 2009, Stockholm

29. Søe M, Holmstrøm K, and Dufva M, Time-laps Study of hybridization kinetics in tissue sections – decreaseing slide to slide variation using flow in microfluidic chambers. COMS 2009 Copenhagen.

28. Sabourin D, Snakenborg D, Dufva M. Peristaltic Micropump for Microfluidic Applications. COMS 2009 Copenhagen.

27. Søe MJ, Møller T, Dufva M, Holmstrøm K, MikroRNA in situ hybridization and immunohistochemical protein detection in formalin-fixed paraffin-embedded tissue sections, 2009 MicroRNA DK

26. Søe MJ, Møller T, Dufva M, Holmstrøm K, MikroRNA in situ hybridization and immunohistochemical protein detection in formalin-fixed paraffin-embedded tissue sections, 2009 4th Danish Conference on Biotechnology and Molecular Biology, Biomarker discovery and drug development in the omics era.

25. Dufva M, Spatial Thermal or Ionic Strength Gradients over DNA Microarray Give Specific and Flexible DNA Microarrays Assays, 2009 Advances in Microarray technology, Stockholm Sweden.

24. Martin Dufva, Jesper Petersen and Lena Poulsen. Increasing the flexibility and reliability of diagnostic and commercial DNA microarrays by microfluidic processing, 2009 Labautomation, Palm Springs, CA.

23. Jesper Petersen, Lena Poulsen, Henrik S. Birgens and Martin Dufva. Salinity gradients for improved array based allele specific hybridization. Advances in microarray technology, 2008, Barcelona

22. Lena Poulsen, Martin Jensen Søe, Lisbeth Birk Moller , Detlef Snakenborg  and Martin Dufva. Signal intensity and specificity of microarray hybridization: Effects of varying the length of spacer and capture probe. Advances in microarray technology, 2008, Barcelona.

21. Haukur Gudnason, Martin Dufva, D.D. Bang, Anders Wolff . An inexpensive and simple method for covalent immobilization of DNA to unmodified glass surfaces for use in thermal cycling reactions. Advances in microarray technology, 2008, Barcelona.

20. David Sabourin, Detlef Snakenborg, Jörg P. Kutter and Martin Dufva. Re-usable PDMS interconnect blocks allowing multiple rapid planar interconnections. Lab-on-a-Chip World Congress, Barcelona, 2008.

19. Blagoi, Gabriela; Keller, Stephan Urs; Dufva, Martin; Boisen, Anja,  Comparison of several methods for chemical modification and micropatterning of the SU-8 photoresist, Presented at: MNE - 33rd International Conference on Micro and Nano Engineering. Copenhagen, Denmark, 2007, In: MNE - 33rd International Conference on Micro and Nano Engineering, p. 283-284,

18. Efficient on-chip real-time PCR using a simple versatile setup, T. Lund-Olesen, M. Dufva, M.F. Hansen. Lab on a chip World congress 2007 Edinburgh

17. Immobilisation of DNA to various substrates by direct UV linking, Haukur Gudnason, Anders Wolff and Martin Dufva, Advances in Microarray technology 2007 Edinburgh

16. T7 amplification for generating single stranded target for hybridization to allele-specific DNA microarrays.- Petersen, J., Poulsen. L., Birgens, H.  and Dufva, M. Advances in microarray technology, 2006, Amsterdam.

15. Dufva, M. “A Multithermal Microfluidic Device for Advanced DNA Microarray Mutation Analysis”, Advances in microarray technology, 2006, Amsterdam. (oral presentation)

14. T. Lund-Olesen, J. Petersen, M. Dufva, and M. F. Hansen. “Capture of DNA in microfluidic channel using magnetic beads: increasing capture efficiency with integrated microfluidic mixer”. Conference on the Scientific and Clinical Applications of Magnetic Carriers. Krems Austria, 2006

13. Michael Stangegaard, Šarūnas Petronis, Claus Bo Vöge Christensen and Martin Dufva. A Biocompatible Micro Cell Culture Chamber (µCCC) for Culturing and On-line Monitoring of Mammalian Cells. Labautomation 2006, Palm Spring, CA, USA

12. Sarunas Petronis, Michael Stangegaard and Martin Dufva: Micromachined polymeric cell culture chamber with uniform media perfussion and integrated temperature control; Proceedings of the 1st Annual Symposium "Life is Matter - Life Matters: Conversations in Bionanotechnology", Göteborg, Sweden, Oct 25-27, 2005, p33

11. Petersen J, Birgens H and Dufva M. “Genotyping â-thalassemia mutation using DNA microarrays detected with an alkaline phosphotase catalysed colour precipitation reaction.”. Advances in Microarray technology, London. Oct 2005.

10. Dufva M, Petersen J, Birgens H and Christensen CBV. “Efficient detection of mutation using microarrays of poly(C)10-poly(T)10 modified DNA probes immobilised on agarose films”. Advances in Microarray technology, London. Oct 2005.

9.   Stangegaard M, Dufva IH, Wohlgehagen V and Dufva M. “Significance of the length of random oligo primers on the yield of cDNA during reverse transcription”. Advances in Microarray technology, London. Oct 2005.

8.   Gudnason H, Wolff A and Dufva M “Immobilization of DNA to polymerized PDMS by direct UV-cross-linking” Advances in Microarray technology, London. Oct 2005.

7.   Microarrays-from research tool to diagnostic necessity. Martin Dufva. Labautomation 2005. San José, CA, USA.

6.   A micro cell culture chamber (µCCC) for the culturing and study of eukaryotic cells. Michael Stangegaard, Sarunas Petronis, Claus B. V. Christensen and Martin Dufva. Screening Europe 2005. Genevé. Switzerland

5.   Marie, R., S. Schmid, et al. (2004). SU-8 cantilevers and one-step functionalization for DNA sensing. Nanotech 2004. The 8th Annual European Conference On Micro & Nanoscale Technologies for the Biosciences Montreux, Switzerland.

4.   Dufva, M., M. Stangegaard, P. Mikkelsen, L. Christensen, and C. Christensen, A flexibel microarray substrate for detecting DNA microarray hybridization using a business card scanner. ALA2004 conferance. Oral Presentation.2004.

3.   Dufva, M., J. Flodin, A. Nerstedt, U. Ruetschi, and L. Rymo, Epstein-Barr Nuclear Antigen 5 inhibits pre-mRNA Cleavage and polyadenylation. EBV2002 Cairns, Australia. Oral presentation.2002.

2.   Dufva, M., A. Nerstedt, and L. Rymo, Repression of Class II Promoters by EBNA5.The 8th International Meeting of the EBV-Association, Stockholm 1998. Poster.1998.

1.   Dufva, M., A. Svenningsson, M. Ahlmkvist, Y.-J. Geng, and G. Hansson, Quantitation of scavenger receptor isoforms by reverse transcription-polymerase chain reaction.VIIIth international symposium on the biology of the vascular wall", Heidelberg. Poster.1994

 

Books and book chapters (9)

9.  Dufva M. Editor of  “Microarrays for biolmedical research” in the Methods Mol Biol  series. 2009.

8.  Dufva M. Introduction to microarray technology. Methods Mol Biol 2009;529:1-22.

7.  Dufva M. Fabrication of DNA microarray. Methods Mol Biol 2009;529:63-79.

6.  Dufva M. Microchips for cell-based assays. Methods Mol Biol 2009;509:135-44.

5.  Dufva M, Christensen CB. Quantification of small molecules using microarray technology. Methods Mol Biol 2007;382:249-58.

4.  Dufva M, Christensen CB. Optimization of oligonucleotide DNA microarrays. Methods Mol Biol 2007;381:93-103.

3.  Dufva M, Poulsen L. Genotyping of mutation in the beta-globin gene using DNA microarrays. Methods Mol Biol 2009;509:47-56.

2.  Petersen J, Poulsen L, Dufva M. Target preparation for genotyping specific genes or gene segments. Methods Mol Biol 2009;529:147-55.

1.  Poulsen L, Petersen J, Dufva M. Genotyping of mutations in the beta-globin gene using allele specific hybridization. Methods Mol Biol 2009;529:157-70.

 

My links

View graph of relations

Publications

(141)

View all »

View all »

  • BioTechniques

    ISSNs: 0736-6205

    Informa Healthcare, United States

    FI (2013): 1, ISI indexed (2013): yes

    Central database

    Journal

  • P L o S One

    ISSNs: 1932-6203, 19326203

    Public Library of Science, United States

    FI (2013): 1, ISI indexed (2013): yes

    Central database

    Journal

  • Lab On a Chip

    ISSNs: 1473-0197, 14730197

    Royal Society of Chemistry, United Kingdom

    FI (2013): 2, ISI indexed (2013): yes

    Central database

    Journal

View all »

Download as:
Download as PDF
Select render style:
ShortLong
PDF
Download as HTML
Select render style:
ShortLong
HTML
Download as Word
Select render style:
ShortLong
Word
Download as: To download, please limit the number of search results to 1,000 or less.

Place of employment

Latest activities and conferences

ID: 2177376