Ditte Hededam Welner

Ditte Hededam Welner

Group Leader

Kemitorvet

Building: 220, 509F

2800 Kgs. Lyngby

Denmark

Mobile: 93513498

Enzyme Engineering and Structural Biology

 

We develop enzyme biocatalysts for the industrial production of natural products used as e.g. aromas, fragrances, dyes, and pharmaceuticals. The existing industrial pipelines have large environmental footprints due to their use of petroleum-derived, hazardous materials and their generation of toxic waste streams. Therefore, replacing the current chemical synthesis routes with bio-based processes  including cell factories and chemo-enzymatic approaches will constitute a significant step towards a greener society.

 

In nature, many natural products are glycosylated by an enzyme class termed secondary metabolism glycosyltransferases (GTs). Glycosylation alters the physico-chemical properties of the natural product, including solubility, stability and bioavailability. We aim at harnessing this glycosylation activity for industrial purposes by tailoring secondary metabolism GTs for specific pipelines. Thus, we are engaged in a systematic study of secondary metabolism GT structural and functional determinants, to provide the framework for efficient secondary metabolism GT discovery and engineering.

 

We combine high-throughput enzyme discovery and evolution with structure-based rational engineering to elucidate the structural determinants of key enzymatic properties such as

 

  • Substrate specificity
  • Enzymatic efficiency
  • Thermostability and pH tolerance

 

Our core methods are X-ray crystallography, enzymology, and other classical protein chemistry techniques for biophysical characterization.

CV

Education

2000 - 2011 Biochemistry - University of Copenhagen

Academic grades

PhD

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  • P L o S One

    ISSNs: 1932-6203

    Additional searchable ISSN (Electronic): 1932-6203

    Public Library of Science, United States

    BFI (2018): BFI-level 1, Scopus rating (2017): CiteScore 3.01 SJR 1.164 SNIP 1.111, ISI indexed (2013): ISI indexed yes, Web of Science (2018): Indexed yes

    Indexed in DOAJ

    Central database

    Journal

  • Acta Crystallographica. Section F: Structural Biology and Crystallization Communications

    ISSNs: 2053-230X

    John Wiley and Sons Ltd, United States

    BFI (2018): BFI-level 1, Scopus rating (2017): CiteScore 0.93 SJR 0.592 SNIP 0.315, ISI indexed (2013): ISI indexed yes, Web of Science (2018): Indexed yes

    Central database

    Journal

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